2005-06-06

(2.50 pm)
- the cheap camera was removed
- plan: to see the projection of the illumination beam to the room ceiling

(2.59 pm)
- can not project easily (at all), a blank microscope slide was placed in between the two objectives

(3.08 pm)
- now, the lower objective was removed
- still, can't project the beam to the ceiling --> maybe I should take the filter out
- a nice circullar beam was observed on the ceiling!  (see Photo_060605_001.jpg)
- the setup is without the two filters and lower objective  (see Photo_060605_002.jpg)

(3.19 pm)
- the lower objective was iserted again
- a spot was seen on the ceiling!  (see Photo_060605_003.jpg and Photo_060605_004.jpg)

- the two filters was placed again --> the spot was disappear!
- one filter was removed --> again, no spot was observed .. even under complete darkness

(3.28 pm)
- the other filter was inserted again 
- a sample 1/20 1um^3 fluorescent bead was prepared (dilution agent = MQ H_2O

(4.00 pm)
- alignment was done using the same trick as yesterday
- an image was obtained (see video file (30 sec): Video_060605_001.3pg)

(4.22 pm)
- a 1/20 1um^3 fluorescent bead sample was made, now the sample was diluted in 1X TAE/Mg++
- (4.27 pm) an image was seen!  on the surface only 
  (see video file (30 sec): Video_060605_001.3pg)
- note that in the movie, the focal plane was changed several time to show that the beads stuck on the surfaces


(4.37 pm)
- I have just realized the obvious consequence of leaving the black box open, the image was not as nice as with the black box closed

(5.38 pm)
- sample= 5uL of (10uL 0.2 uM of UE-H1 tube + 1 uL of cyber gold (concentrated -the idea is that I can always dilute the mix if it's too concentrated) 
- see aggregations of either tube or cyber gold (see Photo_060605_006.jpg and Photo_060605_007.jpg)