2005-06-09

- got a nice Father's day present for my wife (see monitor.jpg)

plan:
(morning)   AFM
(afternoon) spFRET - at least, try to get another image
contact AFM probe sales rep

(9.20)
- a new mica was cleaved and scanned under 1X TAE/2X Mg++ buffer
  (see walker 0609.000-1)

(10.20 am)
- 10uL of each ROO and SOO-H2 were added on mica and let it sit for about 30 min
  (see walker 0609.002-3)

(10.38 am)
- 10uL of H1 was added

(11.07 am)
- image the H1 and H2 complexes
  (see walker 0609.004-5)
  
(11.44 am)
- 4uL of UE-I1 walker was added
- making a movie

AFM parameters
filename=walker0609.
scan size=10.0 um
scan angle=0 deg
scan rate=1.97 Hz
tip velocity 39.4 um/s
drive frequency= 9.93 kHz
Amplitude setpoint= 0.2282 V
Drive amplitude=76.83 mV
Z limit= 1.000 um

Q-control parameter
phase shift= 4.87
feedback amplitude= 1.82
drive amplitude= 8.75

The amplitude signal is much clearer than the topography images

(12.52 pm)
- the movie is still going ... the goal is to take the move until the evaporation finish the buffer
- will ask Harry to analyze the movie

- current verdict --> the walker does not walk!

(1.16 pm)
- evaporation finished the last scan
- 30uL of 1X TAE/1X Mg++ buffer was added on sample and about 10 uL of the 1X TAE/Mg++ was added to the fluid cell.