2005-06-27

Plan:
[X] fix the cables (7.19 am)

[X] image cy3 UE tube
-image FAM SEs tube
-image TAMRA zig-zag

[X] mark
  [X] camera (front, back)
  -translation stage

-level the translation stage 

-start writing the manual
  -camera parameters
  -trick
    -focus using dummy in 2 cover-slips sample
      -dummy = 0.2x 1um bead in either water or 1X TAE/Mg++
    -mark the translation stage

-understand and remove the artifacts
  -find other filters
    -JK’s cheap spec
    -Stratagene filter set
  -rotate optics
-after understand the artifacts, e-mail Bernie

-measure coverslip thickness 
 -compare to 0.17mm for maximum  
 [X] the company says that the thickmess is 0.16-0.19 mm

next:
-try TIRF objective