source: Paul Selvin lab + Harry Choi's lab book

Imaging buffer (50uL)
---------------------
  5uL    of 100mM MgCl_2
  5uL    of 200mM glucose
 10uL    of 5x T50 buffer
  0.5uL  of gloxy (1%)
 29uL    of H_2O
  0.5uL  of \beta-mercaptoethanol

200mM Glucose
-------------
  3.96mg of D-glucose

T50 buffer
----------
(5x)
50mM Tris, pH 8.0, 250 mM NaCl
 69.5mg  of Tris
146mg    of NaCl

100mM MgCl_2 (200uL)
--------------------
 3.95mg of MgCl_2
bring up to 200uL with H_2O

Gloxy
-----
 8.5mg  of glucose oxydase
40.6uL  of catalase
20uL    of 5x T50 buffer
bring up to 100uL with H_2O (39.4 uL)
filter with 0.2um filter
centrifuge for 5 mins at 13k rpm