To do list as 06/16/2005 (rizal) = Myself - learn about optical microscope - source: www.microscopyu.com Fundamentals of light microscopy book - how lens work? = MICROSCOPE [X] understand the current setup with 40x lower objective and cheap camera [X] do all the quick tests that Bernie, Heun Jin, Erik, and Zahid suggested me to do - with the cheap CCD connected to the tv [X] being able to align the microscope quickly - get consistent image with the 40x lower objective o [X] beads [X} SEs tube with 0.1x cyber gold dye [X] FAM SEs tube (TAMRA tube does not form well) - zig-zag - get fluorescence image with 100x objective [X] beads - FAM SEs tube - zig-zag - get fluorescence image with 100x TIRF objective [X] beads - FAM SEs tube - zig-zag - get TIRF working - beads - FAM SEs tube - zig-zag X re-install the Hamamatsu CCD - repeat all the test/imaging with the Hamamatsu CCD = MACHINE SHOP [X] ask access for Psaltis' machine shop [X] install the 2m long synchrotron tube [X] install the revolving nosepiece to the translation stage [X] install the new translation stage for the upper objectives - fix the tilted sample stage - increase the hole for the sample stage = QUAD IMAGER - get more $$$ and purchase quad imager - install the quad imager - multiple wavelengths excitations [ask Bernie] - software to analyze the images = BEAM CHOPPER [ask Bernie] currently, the beam chopper is just a 3M sticky notes = WET LAB - know the protocol to passivate microscope slides - obtain the recipe of the oxygen scavenger buffer [ask Harry] = SOFTWARE - bug other lab members to help me with the software = OTHERS - solve the vibration problem -> move the microscope to ??? - optical table in the AFM room = UTILITIES - collect the catalogs [X] Edmund Scientific - ThorLabs - purchase more lens paper - find/purchase 3 hole covers for the revolving nose piece [X] purchase two missing screws for the black box above the upper objective - purchase optical cleaner from Edmund Scientific - purchase low fluorescence immersion oil from Cargill Inc [X] move gel scanner monitor to storage room